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Sporisorium reilianum 1

Lipase 1

Molecular dynamics simulation 1

Moxifloxacin 1

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Site-directed mutagenesis 1

Sporisorium reilianum 1

biochemical characterization 1

lipase 1

short-chain flavor ester 1

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Efficient Activity Enhancement of a Lipase from Sporisorium reilianum for the Synthesis of a Moxifloxacin Article

Xue Cai, Jiang-Wei Shen, Yu Qiang, Jing Hua, Zhang-Qi Ma, Zhi-Qiang Liu, Yu-Guo Zheng

Engineering 2022, Volume 19, Issue 12, Pages 207-216 doi: 10.1016/j.eng.2022.03.020

Abstract: In our previous study, a lipase from Sporisorium reilianum (SRL) was identified to possess excellent

Keywords： Lipase Sporisorium reilianum Site-directed mutagenesis Molecular dynamics simulation Rational

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Expression and characterization of a CALB-type lipase from

Jiang-Wei Shen, Xue Cai, Bao-Juan Dou, Feng-Yu Qi, Xiao-Jian Zhang, Zhi-Qiang Liu, Yu-Guo Zheng

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 5, Pages 868-879 doi: 10.1007/s11705-019-1889-x

Abstract: A lipase from SRZ2 (SRL) with 73% amino acid sequence identity to lipase B (CALB) was cloned and overexpressed in . The recombinant SRL showed a preference for short-chain -nitrophenyl esters. It achieved maximum activity at pH 8.0 and 65°C for -nitrophenyl hexanoate (C6) with and / values of 0.14 mmol∙L and 1712 min ∙mmol∙L at 30°C, respectively. SRL displayed excellent thermostability and pH stability, retaining more than 79% of its initial activity after incubation at 60°C for 72 h and 75% at pH 3 to 11 for 72 h. It also maintained most of its activity in the presence of inhibitors and detergents except sodium dodecyl sulfate, and it tolerated organic solvents. SRL was covalently immobilized and successfully used for ethyl hexanoate synthesis in cyclohexane or in a solvent-free system with a high conversion yield (>95%). Furthermore, high conversion yield was also achieved for the synthesis of various short-chain flavor esters when high substrate concentrations of 2 mol∙L were applied. This study indicated that a CALB-type lipase from SRZ2 showed great potential in organic ester synthesis.

Keywords： lipase Sporisorium reilianum biochemical characterization short-chain flavor ester solvent-free system